plaque forming unit,PF[J

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问题 plaque forming unit,PF[J

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答案plaque forming unit,PFU:The plaque assay is the most widly used assay for infectious virus.Monolayers ofhost cells are inoculated with suitable dilutions of virusand affter adsorption aye overlaid with medium containingagar or carboxyme thyicellulose to prevent virus spreadingthroughout the culture.Affter several days,the cell s inital—ly infected have produced virus that spreads only to sur—rounding cells,producing a small area of infection or plaque.Under controlled conditions,a single plaque can arise form a single infectious virus particle,termed aplaque—forming unit.

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