首页
外语
计算机
考研
公务员
职业资格
财经
工程
司法
医学
专升本
自考
实用职业技能
登录
外语
Dear sir, Thank you for your kind letter of December 11, 2003. We are happy to know that our paper entitled "Purification o
Dear sir, Thank you for your kind letter of December 11, 2003. We are happy to know that our paper entitled "Purification o
admin
2010-01-10
64
问题
Dear sir,
Thank you for your kind letter of December 11, 2003. We are happy to know that our paper entitled "Purification of Clostridium perfringens phospholipas C (@-toxin) by affinity chromatography on agarose-liked egg-yolk lipoprotein" (Art no. RPP-1265) will be acceptable. We have tried to shorten and revise the manuscript in line with the suggestion made by one of the reviewers and yourself. I am enclosing duplicate copies of the revised version. In answer to the questions raised by the reviewer, we must admit that we have not tested for lipase activity known to exist in culture of this organism. However, since our purified phospholipase C was found homogeneous by various criteria, we believe contamination with lipase, if there is any, is likely to be slight. In any case, we will test for lipase activity as soon as possible.
As to the second question, we realized that the lipoprotein in affinity adsorbent was attacked by the enzyme to a small extent; a minute amount of phosphorycholine was always detected in the break-through peak. As reported, however, the same column can be repeatedly (at least four times) without losing its affinity for the enzyme.
We still do not know whether or not all of the multiple forms of enzyme are artifacts formed exclusively during isoeletric focusing. But some evidence is now available for the presence of at least two forms of enzyme, which are separable by methods other than isoeletric focusing, i. e. CM--or DEAE--Sephadex chromatography (unpublished data). The clarification of this question is now in progress in our laboratory.
We have accepted all the suggestions penciled in by the reviewer on the original manuscript; the abbreviation SDC has been avoided. Through these revision we have succeeded in shortening the manuscript by two pages in total, although the page numbers have been kept unchanged. In the revised manuscript we have put the reference numbers in the right order as requested. We would like, however, to keep the designation of the figures as it was, since we believe the suggestion may be based on a misunderstanding on the part of reviewers. As suggested, we have improved the description of the essential step involved in preparation of the affinity adsorbed (page 6,5th line from the bottom of the original manuscript) as follows; "by centrifuging the mixture at 13,000g for 15 min. to discard the precipitate." I hope that these revisions and the shortened text are satisfactory and that the revised version will be acceptable for publication in Biochimica et Biophysica Acta. I also hope the revised manuscript will reach you before January 1.
Sincerely yours,
Nobuo Ogata, M.D.
The author is likely to major in ______.
选项
A、biology
B、physics
C、geology
D、journalism
答案
A
解析
转载请注明原文地址:https://kaotiyun.com/show/xDKd777K
本试题收录于:
BEC高级阅读题库BEC商务英语分类
0
BEC高级阅读
BEC商务英语
相关试题推荐
WhattypeofbusinessdoesJackWilliamsown?
Whatistheproblem?
Whattypeofbusinessisbeingadvertised?
A、 B、 C、 B(A)疑问词引导的疑问句不可以用yes/no来回答,并且,重复使用president一词,易造成混淆。(B)针对询问谁会当选副总裁的提问,做出了应该静候结果的回答,故为正确答案。(C)重复使用pres
Howlongdoestheprocessofinternationalshippingdocumentstake?
Whatisthereasonfortheflightdelay?
Whatpositionarethecandidatesapplyingfor?
Isadvertisingimportantornot?Whatfactorsmustonetakeintoaccountwhendevelopingadvertisingstrategy?
•YouwillheartheChairmanoftheBusinessBroadcastingCommitteeofatelevisioncompany.Heisaddressingameetingofthec
•Youwillhearpartofaconversationbetweentwocompanyemployees,awomancalledRoseandamancalledSteve.•Foreachque
随机试题
班级管理
骨科常用的成人跟骨牵引重量为
可确诊疑是结核性腹膜炎的检查为
A.非限制使用级抗菌药物B.限制使用级抗菌药物C.特殊使用级抗菌药物D.特殊限制使用级抗菌药物病原菌只对限制使用级抗菌药物敏感时可选用()。
一8月大健康婴儿,先采用人工喂养,家长到儿保门诊咨询喂养方法,此时护士应指导添加的辅食是
当()时,证券公司及其营业部可以协助期货公司向客户提示风险。
以应收账款出质的,质权自()设立。
甲公司欲购乙公司生产的塔吊,因缺乏资金,遂由丙公司提供融资租赁。由于塔吊存在质量问题,吊装的物品坠落并砸伤行人丁,甲公司被迫停产修理。根据合同法律制度的规定,下列各项中,正确的有()。
什么是幼儿园环境?为什么幼儿园教育中要强调创设良好的幼儿园环境?请联系实际说明。
在“佛山小悦悦”事件中的那些围观者,剥离了冷漠的标签,也是普通的民众。他们未尝就不懂,没有人是座孤岛,相互搀扶才能同舟共济;“乐于助人”是传统的人伦美德,漠视生命,会让良知溃散。或许换个语境,他们也会痛斥“铁石心肠”,呼吁人性回归。可一旦轮到自己做“路人甲
最新回复
(
0
)